Plekha1^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Plekha1^{tm1a(EUCOMM)Hmgu}
• Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5501548
• Gene: Plekha1 Location: Chr7:130467486-130515042 bp, + strand Genetic Position: Chr7, 73.19 cM
• Alliance: Plekha1^{tm1a(EUCOMM)Hmgu} page
• IMPC: Plekha1 gene page

Mutation origin

• Mutant Cell Lines: HEPD0897_7_A02, HEPD0897_7_A03, HEPD0897_7_A04, HEPD0897_7_A05, HEPD0897_7_A07, HEPD0897_7_A08, HEPD0897_7_A09, HEPD0897_7_A11, HEPD0897_7_B01, HEPD0897_7_B02, HEPD0897_7_B03, HEPD0897_7_B04, HEPD0897_7_B05, HEPD0897_7_B06, HEPD0897_7_B07, HEPD0897_7_B09, HEPD0897_7_B10, HEPD0897_7_B11, HEPD0897_7_C03, HEPD0897_7_C04, HEPD0897_7_C07, HEPD0897_7_C08, HEPD0897_7_C09, HEPD0897_7_C10, HEPD0897_7_C11, HEPD0897_7_D01, HEPD0897_7_D02, HEPD0897_7_D03, HEPD0897_7_D04, HEPD0897_7_D05, HEPD0897_7_D06, HEPD0897_7_D07, HEPD0897_7_D08, HEPD0897_7_D09, HEPD0897_7_D11, HEPD0897_7_D12, HEPD0897_7_E01, HEPD0897_7_E02, HEPD0897_7_E03, HEPD0897_7_E04, HEPD0897_7_E06, HEPD0897_7_E07, HEPD0897_7_E08, HEPD0897_7_E10, HEPD0897_7_E11, HEPD0897_7_F02, HEPD0897_7_F03, HEPD0897_7_F04, HEPD0897_7_F05, HEPD0897_7_F07, HEPD0897_7_F08, HEPD0897_7_F09, HEPD0897_7_F10, HEPD0897_7_F11, HEPD0897_7_F12, HEPD0897_7_G01, HEPD0897_7_G02, HEPD0897_7_G03, HEPD0897_7_G04, HEPD0897_7_G05, HEPD0897_7_G06, HEPD0897_7_G07, HEPD0897_7_G08, HEPD0897_7_G09, HEPD0897_7_G11, HEPD0897_7_H01, HEPD0897_7_H03, HEPD0897_7_H04, HEPD0897_7_H05, HEPD0897_7_H06, HEPD0897_7_H08, HEPD0897_7_H11, HEPD0897_7_H12
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_GT0_LF2A_LacZ_BetactP_neo
• Mutation details: The L1L2_GT0_LF2A_LacZ_BetactP_neo cassette was inserted at position 130506405 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 130506810. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Plekha1 Mutation: 54 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)