Ralb^tm1.2Cjm
Targeted Allele Detail

Summary

• Symbol: Ralb^tm1.2Cjm
• Name: v-ral simian leukemia viral oncogene B; targeted mutation 1.2, Christopher J Marshall
• MGI ID: MGI:5505280
• Gene: Ralb Location: Chr1:119398035-119432512 bp, - strand Genetic Position: Chr1, 52.29 cM, cytoband E2
• Alliance: Ralb^tm1.2Cjm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:199742
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette was inserted downstream of exon 2. Flp-mediated recombination removed the neomycin resistance cassette and left exon 2 floxed. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts, kidney, lung and testis. (J:199742)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ralb Mutation: 47 strains or lines available

References

• Original: J:199742 Peschard P, et al., Genetic deletion of RALA and RALB small GTPases reveals redundant functions in development and tumorigenesis. Curr Biol. 2012 Nov 6;22(21):2063-8
• All: 4 reference(s)