Snap25^{tm2.1(cre)Hze}
Targeted Allele Detail

Summary

• Symbol: Snap25^{tm2.1(cre)Hze}
• Name: synaptosomal-associated protein 25; targeted mutation 2.1, Hongkui Zeng
• MGI ID: MGI:5507846
• Synonyms: Snap25-IRES2-Cre, Snap25-IRES2-Cre-D, Snap25-IRES2-Cre-Delta, Snap25-IRES2-Cre-DeltaNeo/Hygro, Snap25^{m1(IRES2-Cre)Hze}
• Gene: Snap25 Location: Chr2:136555373-136624348 bp, + strand Genetic Position: Chr2, 67.56 cM
• Alliance: Snap25^{tm2.1(cre)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146821
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Snap25^{tm2.1(cre)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Snap25 (MGI:98331)

• Mutation details: The targeting vector contained, from 5' to 3', a partial Snap25 sequence spanning intron 6 through intron 7, an frt3 site within Snap25 intron 7, a partial Snap25 exon 8 sequence up to and including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence, a cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor, the 3' portion of the hygromycin gene with SV40 late polyA signal, and an AttP site. This construct was electroporated into G4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to PhiC31-expressing mice to remove the AttB/AttP-flanked sequences (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replace it with the recombined AttB/AttP site (AttL). The resulting Snap25-IRES2-Cre-D mice were bred with C57BL/6J wildtype mice to remove the PhiC31 transgene. (J:146821, J:220523)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Snap25 (mouse) Summary of all recombinase alleles driven by Snap25. Alliance mouse Snap25 gene page
User Notes:	Additional information submitted by the research community Additional information submitted by the research community Data for AIBS characterization of recombinase activity: \link(Snap25-IRES2-Cre images)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Snap25 Mutation: 26 strains or lines available

References

• Original: J:220523 Harris JA, et al., Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation. Front Neural Circuits. 2014;8:76
• All: 17 reference(s)