Usp30^{tm2a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Usp30^{tm2a(EUCOMM)Hmgu}
• Name: ubiquitin specific peptidase 30; targeted mutation 2a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5511766
• Gene: Usp30 Location: Chr5:114238395-114261571 bp, + strand Genetic Position: Chr5, 55.96 cM
• Alliance: Usp30^{tm2a(EUCOMM)Hmgu} page
• IMPC: Usp30 gene page

Mutation origin

• Mutant Cell Lines: HEPD0810_8_A05, HEPD0810_8_A06, HEPD0810_8_B07, HEPD0810_8_B08, HEPD0810_8_C05, HEPD0810_8_C06, HEPD0810_8_C07, HEPD0810_8_D06, HEPD0810_8_D08, HEPD0810_8_E05, HEPD0810_8_E06, HEPD0810_8_E07, HEPD0810_8_E08, HEPD0810_8_F06, HEPD0810_8_F07, HEPD0810_8_F08, HEPD0810_8_G06, HEPD0810_8_G07, HEPD0810_8_G08, HEPD0810_8_H05, HEPD0810_8_H07, HEPD0810_8_H08
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1.C2 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 114243487 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 114244160. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Usp30 Mutation: 34 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)