Arap2^{Gt(PT1-ATG)9AVal}
Gene trapped Allele Detail

Summary

• Symbol: Arap2^{Gt(PT1-ATG)9AVal}
• Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2; gene trap mutation 9A, Katherine A Vallis
• MGI ID: MGI:5514159
• Synonyms: 9A
• Gene: Arap2 Location: Chr5:62759788-62923502 bp, - strand Genetic Position: Chr5, 32.04 cM
• Alliance: Arap2^{Gt(PT1-ATG)9AVal} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:197093
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: The PT1-ATG gene trap vector has integrated into the endogenous gene immediately downstream of bp 4622. This vector contains the engrailed 2 (En2) splice acceptor sequence joined to a promoterless lacZ (E. coli beta-galactosidase) reporter gene with an ATG translation initiation codon; integration of the construct into an intron results in transcription of a fusion mRNA containing the 5' exons of the trapped gene joined to the lacZ coding sequence. PT1-ATG also contains the neomycin phosphotransferase gene under control of the phosphoglycerate kinase 1 (PGK1) promoter (PGK1-neo). The 9A ES cell clone was selected for chimera production because it exhibited significantly elevated beta-galactosidase activity following exposure to gamma radiation. (J:82809, J:197093)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Arap2 Mutation: 104 strains or lines available

References

• Original: J:197093 Vallis KA, et al., Identification of radiation-responsive genes in vitro using a gene trap strategy predicts for modulation of expression by radiation in vivo. Radiat Res. 2002 Jan;157(1):8-18
• All: 2 reference(s)