Ehftm1a(EUCOMM)Hmgu
Targeted Allele Detail
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Symbol: |
Ehftm1a(EUCOMM)Hmgu |
Name: |
ets homologous factor; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH |
MGI ID: |
MGI:5520288 |
Gene: |
Ehf Location: Chr2:103093776-103133620 bp, - strand Genetic Position: Chr2, 54.43 cM
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Alliance: |
Ehftm1a(EUCOMM)Hmgu page
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IMPC: |
Ehf gene page |
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Mutant Cell Lines: |
HEPD0932_6_A02, HEPD0932_6_B02, HEPD0932_6_B04, HEPD0932_6_C01, HEPD0932_6_C03, HEPD0932_6_F01, HEPD0932_6_F03, HEPD0932_6_G01 |
Germline Transmission: |
Earliest citation of germline transmission:
J:204812
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo
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Mutation details: The Ifitm2_intron_L1L2_GT0_LF2A_LacZ_BetactP_neo cassette was inserted at position 103109665 of Chromosome 2 (Build GRCm39) within an endogenous exon, creating an artificial intron that splits the original exon into two artificial exons, where the downstream artificial exon is critical. The cassette is composed of the intron of the Ifitm2 gene containing an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 103109978. The critical artificial exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other EUCOMMTOOLs alleles can be found at http://www.knockoutmouse.org/about/eucommtools/vectors.
(J:157065)
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Original: |
J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015; |
All: |
2 reference(s) |
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