Mthfr^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Mthfr^{tm1a(EUCOMM)Hmgu}
• Name: methylenetetrahydrofolate reductase; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5521869
• Gene: Mthfr Location: Chr4:148123534-148144008 bp, + strand Genetic Position: Chr4, 78.67 cM
• Alliance: Mthfr^{tm1a(EUCOMM)Hmgu} page
• IMPC: Mthfr gene page

Mutation origin

• Mutant Cell Lines: HEPD0940_4_A10, HEPD0940_4_A12, HEPD0940_4_B11, HEPD0940_4_C05, HEPD0940_4_C09, HEPD0940_4_C11, HEPD0940_4_D06, HEPD0940_4_D07, HEPD0940_4_D10, HEPD0940_4_D11, HEPD0940_4_E05, HEPD0940_4_E08, HEPD0940_4_F06, HEPD0940_4_F09, HEPD0940_4_F10, HEPD0940_4_G06, HEPD0940_4_H04
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 148127397 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 148128352. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Mthfr Mutation: 70 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)