Vamp3^{tm2a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Vamp3^{tm2a(EUCOMM)Wtsi}
• Name: vesicle-associated membrane protein 3; targeted mutation 2a, Wellcome Trust Sanger Institute
• MGI ID: MGI:5527590
• Gene: Vamp3 Location: Chr4:151131762-151142410 bp, - strand Genetic Position: Chr4, 81.53 cM, cytoband E1
• Alliance: Vamp3^{tm2a(EUCOMM)Wtsi} page
• IMPC: Vamp3 gene page

Mutation origin

• Mutant Cell Lines: MEPD1004_2_A03, MEPD1004_2_A08, MEPD1004_2_A10, MEPD1004_2_B04, MEPD1004_2_B06, MEPD1004_2_B10, MEPD1004_2_C02, MEPD1004_2_C09, MEPD1004_2_C10, MEPD1004_2_D03, MEPD1004_2_D05, MEPD1004_2_D08, MEPD1004_2_E01, MEPD1004_2_E02, MEPD1004_2_E04, MEPD1004_2_E07, MEPD1004_2_F03, MEPD1004_2_F07, MEPD1004_2_F11, MEPD1004_2_G01, MEPD1004_2_G03, MEPD1004_2_G04, MEPD1004_2_G05, MEPD1004_2_G09, MEPD1004_2_H01, MEPD1004_2_H03, MEPD1004_2_H07, MEPD1004_2_H11
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1.C2 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 151140369 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 151141121. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Vamp3 Mutation: 17 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 3 reference(s)