Rapgef3^tm1.1Chen
Targeted Allele Detail

Summary

• Symbol: Rapgef3^tm1.1Chen
• Name: Rap guanine nucleotide exchange factor (GEF) 3; targeted mutation 1.1, Ju Chen
• MGI ID: MGI:5559594
• Synonyms: Epac1^-
• Gene: Rapgef3 Location: Chr15:97642651-97665853 bp, - strand Genetic Position: Chr15, 53.74 cM
• Alliance: Rapgef3^tm1.1Chen page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:207599
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 3. A neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 5. Cre-mediated recombination removed exons 2 through 5. Western blot analysis confirmed the absence of protein expression in the heart, liver, pancreas and kidney. (J:207599)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rapgef3 Mutation: 47 strains or lines available

References

• Original: J:207599 Yan J, et al., Enhanced leptin sensitivity, reduced adiposity, and improved glucose homeostasis in mice lacking exchange protein directly activated by cyclic AMP isoform 1. Mol Cell Biol. 2013 Mar;33(5):918-26
• All: 11 reference(s)