Tg(Csk*)2Weis
Transgene Detail

Summary

• Symbol: Tg(Csk*)2Weis
• Name: transgene insertion 2, Arthur Weiss
• MGI ID: MGI:5563915
• Synonyms: Csk^AS
• Transgene: Tg(Csk*)2Weis Location: unknown
• Alliance: Tg(Csk*)2Weis page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Csk*)2Weis expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Csk (MGI:88537)

• Mutation details: A bacterial artificial chromosome (BAC) library from CHORI was used to obtain a BAC (#RP24-400B5) containing the entire mouse Csk (c-src tyrosine kinase) gene. A point mutation was introduced in Csk at position 266 resulting in a threonine to glycine mutation, inducing a larger ATP-binding pocket to be formed in the expressed protein. To inactivate other genes present in the BAC, both ends of the BAC were deleted, leaving 74kb of sequence, and an in-frame stop codon was inserted in exon 2 of Lman1l (lectin, mannose-binding 1 like) gene. (J:208674)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:208674 Tan YX, et al., Inhibition of the kinase Csk in thymocytes reveals a requirement for actin remodeling in the initiation of full TCR signaling. Nat Immunol. 2014 Feb;15(2):186-94
• All: 8 reference(s)