Tg(ACTA1-Utrn)3Ked
Transgene Detail

Summary

• Symbol: Tg(ACTA1-Utrn)3Ked
• Name: transgene insertion 3, Kay E Davies
• MGI ID: MGI:5566891
• Synonyms: Freddie, Fre line, TgN(FLU)Ox(Freddie)
• Transgene: Tg(ACTA1-Utrn)3Ked Location: unknown
• Alliance: Tg(ACTA1-Utrn)3Ked page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA/Ca)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(ACTA1-Utrn)3Ked expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  Not Specified	Utrn

• Mutation details: This transgene contains the promoter and regulatory sequences of the human actin, alpha 1, skeletal muscle (ACTA1) gene driving expression of a full-length utrophin cDNA with the SV40 large T antigen polyadenylation signal. Western blot analysis of extracts of tibialis anterior (TA), extensor digitalis longus (EDL) and diaphragm muscle using a utrophin-specific monoclonal antibody (MANCHO3) demonstrates utrophin expression in these muscles of transgenic Dmd^mdx/Dmd^mdx mice at similar or lower levels than the normally high level expression from the endogenous gene in lung and kidney. Immunohistoluminescent analysis reveals relocalization of utrophin to the sarcolemma of transgenic Dmd^mdx/Dmd^mdx tibialis anterior muscle. (J:51364)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

This is one of three transgenic lines reported. Their relative levels of utrophin expression from the transgene in diaphragm and tibialis anterior muscle are Tg(ACTA1-Utrn)2Ked (a.k.a. Fiona, Fio) > Tg(ACTA1-Utrn)1Ked (Fergie, Fer) > Tg(ACTA1-Utrn)3Ked (Freddie, Fre). (J:51364)

References

• Original: J:51364 Tinsley J, et al., Expression of full-length utrophin prevents muscular dystrophy in mdx mice. Nat Med. 1998 Dec;4(12):1441-4
• All: 1 reference(s)