Gt(ROSA)26Sor^{tm1(CAG-mCherry/Rpl10a)Sten}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-mCherry/Rpl10a)Sten}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Jan M Stenman
• MGI ID: MGI:5569759
• Synonyms: Gt(ROSA)26Sor-mCherry-Rpl10a, mCherryTRAP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-mCherry/Rpl10a)Sten} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:210222
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The "floxed translating ribosome affinity purification (TRAP)" insertion encodes an mCherry-tagged ribosomal protein L10A, which incorporates into and fluorescently labels polysomes in cell lineages determined by the cell type-specific expression of cre recombinase. As mRNAs encoding expressed proteins are attached to polysomes during their translation, the transcripts active in a cell or lineage at times from the onset of cre expression can thus be identified. The introduced segment contains an mRNA splice acceptor sequence followed by a puromycin resistance cassette and polyadenylation signal; the CAG promoter, comprising the cytomegalovirus (CMV) immediate ealy (IE) enhancer and chicken beta-actin promoter, blocked by a loxP-flanked triple SV40 polyadenylation signal (tpA); and the mCherry coding sequence joined to the 5' end of that for ribosomal protein L10A (encoded by the Rlp10a gene), followed by the rabbit beta-globin polyadenylation signal. Cre recombinase excision of the floxed tpA permits expression of the fusion protein under control of the ubiquitous CAG enhancer/promoter. The selection of the cre recombinase transgene or knockin allele thus provides spatiotemporal control of the expression of the fluorescently tagged L10A protein. (J:210222)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1006 strains or lines available

References

• Original: J:210222 Hupe M, et al., Evaluation of TRAP-sequencing technology with a versatile conditional mouse model. Nucleic Acids Res. 2014 Jan;42(2):e14
• All: 7 reference(s)