Cdkl5^tm1.2Cogr
Targeted Allele Detail

Summary

• Symbol: Cdkl5^tm1.2Cogr
• Name: cyclin dependent kinase like 5; targeted mutation 1.2, Cornelius Gross
• MGI ID: MGI:5573238
• Synonyms: Cdkl5KO
• Gene: Cdkl5 Location: ChrX:159567241-159777673 bp, - strand Genetic Position: ChrX, 73.95 cM
• Alliance: Cdkl5^tm1.2Cogr page

Hind-limb clasping in Cdkl5^tm1.2Cogr/Cdkl5^tm1.2Cogr mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209635
• Parent Cell Line: A8 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: Exon 4, which was flanked by loxP sites 806 bp upstream and 347 bp downstream of the exon, has been deleted by cre recombinase, and a FRT-flanked neomycin resistance cassette that followed the second loxP site was deleted by flp recombinase. CDKL5 protein was shown to be present at reduced levels in female mice heterozygous for the mutation and absent in homozygous mutant female and hemizygous male mice by immunoblot analysis of whole brain extracts and immunofluorescent imaging of brain sections. (J:209635) Inheritance: Semidominant

Generation of the Cdkl5^tm1Cogr, Cdkl5^tm1.1Cogr, and Cdkl5^tm1.2Cogr alleles

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cdkl5 Mutation: 20 strains or lines available

Notes

According to EMMA, the parental ES cell line is A9, although ES cell line A8 was given in the original publication J:209635.

References

• Original: J:209635 Amendola E, et al., Mapping Pathological Phenotypes in a Mouse Model of CDKL5 Disorder. PLoS One. 2014;9(5):e91613
• All: 25 reference(s)