Gt(ROSA)26Sor^{tm1.1(CAG-cas9*,-EGFP)Fezh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-cas9*,-EGFP)Fezh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Feng Zhang
• MGI ID: MGI:5583838
• Synonyms: constitutive Cas9, Gt(ROSA)26Sor^{tm1.1(CAG-cas9,-EGFP)Fezh}, Rosa26-Cas9
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-cas9*,-EGFP)Fezh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:213550
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Endonuclease, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector inserted into the locus was designed with (from 5' to 3') a 5' homology arm, a ubiquitously expressed CAG promoter, loxP-flanked 3xSV40 polyA stop cassette, a 3X-FLAG epitope tag, a mammalian codon-optimized cas9 gene (derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]) flanked by two nuclear localization signals, a P2A ribosomal skip cleavage peptide sequence, an enhanced Green Fluorescent Protein (EGFP) gene, a woodchuck hepatitis virus post-transcriptional regulatory element, bovine growth hormone polyA, pPGK-Neo-pA positive selection cassette and a 3' homology arm. Cre-mediated recombination removed the floxed STOP cassette. (J:213550)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:213550 Platt RJ, et al., CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling. Cell. 2014 Oct 9;159(2):440-55
• All: 135 reference(s)