Mlkl^tm1.2Wsa
Targeted Allele Detail

Summary

• Symbol: Mlkl^tm1.2Wsa
• Name: mixed lineage kinase domain-like; targeted mutation 1.2, Warren S Alexander
• MGI ID: MGI:5614464
• Gene: Mlkl Location: Chr8:112038429-112064809 bp, - strand Genetic Position: Chr8, 57.98 cM, cytoband D3
• Alliance: Mlkl^tm1.2Wsa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:204620
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A FRT site flanked neomycin resistance cassette was inserted in intron 3 along with loxP sites upstream of exon 3 and immediately downstream of the resistance cassette. The neo cassette was removed via Flp-mediated recombination and the loxP flanked exon 3 was deleted via Cre-mediated recombination. Western blot analysis confirmed absence of protein in all tissues examined. (J:204620)

Expression

In Mice Carrying this Mutation:

2 assay results 3 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mlkl Mutation: 32 strains or lines available

References

• Original: J:204620 Murphy JM, et al., The pseudokinase MLKL mediates necroptosis via a molecular switch mechanism. Immunity. 2013 Sep 19;39(3):443-53
• All: 87 reference(s)