Slc25a21^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc25a21^{tm1d(KOMP)Wtsi}
• Name: solute carrier family 25 (mitochondrial oxodicarboxylate carrier), member 21; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5629856
• Gene: Slc25a21 Location: Chr12:56759419-57244257 bp, - strand Genetic Position: Chr12, 24.54 cM
• Alliance: Slc25a21^{tm1d(KOMP)Wtsi} page
• IMPC: Slc25a21 gene page

Mutation origin

• Mutant Cell Line: EPD0085_1_D04
• Germline Transmission: Earliest citation of germline transmission: J:215078
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 56821858 of Chromosome 12 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 56822635. The critical exon 4 is thus flanked by loxP sites. Flp recombinase expression removed the selection cassette. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:215078)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc25a21 Mutation: 26 strains or lines available

References

• Original: J:215078 Maguire S, et al., Targeting of Slc25a21 is associated with orofacial defects and otitis media due to disrupted expression of a neighbouring gene. PLoS One. 2014;9(3):e91807
• All: 4 reference(s)