Slc25a21^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc25a21^{tm1c(KOMP)Wtsi}
• Name: solute carrier family 25 (mitochondrial oxodicarboxylate carrier), member 21; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5629861
• Gene: Slc25a21 Location: Chr12:56759419-57244257 bp, - strand Genetic Position: Chr12, 24.54 cM
• Alliance: Slc25a21^{tm1c(KOMP)Wtsi} page
• IMPC: Slc25a21 gene page

Mutation origin

• Mutant Cell Line: EPD0085_1_D04
• Germline Transmission: Earliest citation of germline transmission: J:215078
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 56821858 of Chromosome 12 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 56822635. The critical exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:215078)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slc25a21 Mutation: 26 strains or lines available

References

• Original: J:215078 Maguire S, et al., Targeting of Slc25a21 is associated with orofacial defects and otitis media due to disrupted expression of a neighbouring gene. PLoS One. 2014;9(3):e91807
• All: 1 reference(s)