Tg(Rnu6-RNAi:IE-PRV)SH05Gjo
Transgene Detail

Summary

• Symbol: Tg(Rnu6-RNAi:IE-PRV)SH05Gjo
• Name: transgene insertion SH05, Genevieve Jolivet
• MGI ID: MGI:5645892
• Synonyms: SH05
• Transgene: Tg(Rnu6-RNAi:IE-PRV)SH05Gjo Location: unknown
• Alliance: Tg(Rnu6-RNAi:IE-PRV)SH05Gjo page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic
• Mutation: Insertion
• Mutation details: The U6-shRNA construct encompasses the U6 mouse promoter that drives the transcription of a shRNA expressing transcription unit. The shRNA sequence targets the 3'-part of the intermediate early gene (IE gene) of the Pseudorabies virus (PRV), with the following sequence (5'-CTCCCATCGGGTGAGAAAA-3'). The Pseudorabies Virus (PRV) infects currently farm animals (especially pigs) and mice. The U6-shRNA gene is inserted in an empty transgene expression vector encompassing the 5'HS4 insulator from the chicken beta globin gene locus, the human eF1alpha gene promoter (-1430, +2), the rabbit (rb) beta-globin second intron and several enhancers (E1, E2 and E3). The empty expression vector acts as a cargo for the U6-shRNA gene to insure the reliable expression. Transgenic lines SHO3, SHO5 and SH30 were created. All lines are resistant to PRV viral infection. (J:211915)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:211915 Daniel-Carlier N, et al., Viral infection resistance conferred on mice by siRNA transgenesis. Transgenic Res. 2013 Jun;22(3):489-500
• All: 1 reference(s)