Tg(tetO-Creb*)95Brco
Transgene Detail

Summary

• Symbol: Tg(tetO-Creb*)95Brco
• Name: transgene insertion 95, Angel Barco
• MGI ID: MGI:5648355
• Synonyms: AC95, Tg(tetO-ACREB)95
• Transgene: Tg(tetO-Creb*)95Brco Location: unknown
• Alliance: Tg(tetO-Creb*)95Brco page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-Creb*)95Brco expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Creb1 (MGI:88494)

• Mutation details: The inducible transgene expresses a dominant-negative inhibitor of CREB, termed A-CREB, by replacing the CREB basic region with an acidic amphipathic protein sequence. The DNA construct was designed to express the following: the first 8 amino acids encode the FLAG epitope tag, the next 13 amino acids are a phi10 protein sequence, the next 31 amino acids are the amphipathic acidic extension, and the final group of amino acids is the mouse CREB leucine zipper, which continues to the natural C terminus of the protein. The mouse CREB B-ZIP domain spans from Leu-274 to Asp-341, the natural C terminus. cDNAs encoding the different B-ZIP leucine zippers and a stop codon were generated by PCR and ligated to sequences encoding the C terminus of the acidic extension. (J:211460)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:211460 Jancic D, et al., Inhibition of cAMP response element-binding protein reduces neuronal excitability and plasticity, and triggers neurodegeneration. Cereb Cortex. 2009 Nov;19(11):2535-47
• All: 1 reference(s)