Ifnar1^tm1.2Ees
Targeted Allele Detail

Summary

• Symbol: Ifnar1^tm1.2Ees
• Name: interferon (alpha and beta) receptor 1; targeted mutation 1.2, Edward E Schmidt
• MGI ID: MGI:5692167
• Gene: Ifnar1 Location: Chr16:91282126-91304329 bp, + strand Genetic Position: Chr16, 52.98 cM
• Alliance: Ifnar1^tm1.2Ees page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229084
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (C57BL/6 x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting vector was designed to insert a self-excising FRT-Prm1pFlppol2Neo-FRT cassette and loxP site in intron 2, and a second loxP site in intron 3 of the gene. Facilitated self-excision of the cassette in the male germline and resulted in offspring with one FRT site and loxP site upstream of exon 3, and a second loxP site downstream of exon 3. Cre-mediated recombination removed the floxed neo cassette and exon 3. The resulting non-functional protein contains an exon 2-4 splice variant and frameshift leading to translation of 11 arbitrary (missense) amino acids within the N-terminal extracellular domain followed by a stop codon. (J:229084)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Ifnar1 Mutation: 60 strains or lines available

References

• Original: J:229084 Prigge JR, et al., Type I IFNs Act upon Hematopoietic Progenitors To Protect and Maintain Hematopoiesis during Pneumocystis Lung Infection in Mice. J Immunol. 2015 Dec 1;195(11):5347-57
• All: 141 reference(s)