Gt(ROSA)26Sor^{tm1(CAG-Chrm3*/GFP,cAMPRE-luc)Berd}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Chrm3*/GFP,cAMPRE-luc)Berd}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Rebecca Berdeaux
• MGI ID: MGI:5696731
• Synonyms: ROSA26^GsD, ROSA26-GsD-luc
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Chrm3*/GFP,cAMPRE-luc)Berd} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239137
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct inserted into the locus a CAG promoter; floxed STOP cassette; an HA tagged mutant G protein-coupled receptor (GPCR) sequence of the Galpha^s-coupled rat muscarinic 3 DREADD (designer receptor exclusively activated by designer drug), also referred to as R-s; a GFP reporter gene; and a cAMP responsive element activated by cAMP that drives expression of a luciferase reporter gene. (J:239137)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 997 strains or lines available

References

• Original: J:239137 Akhmedov D, et al., Gs-DREADD Knock-In Mice for Tissue-Specific, Temporal Stimulation of Cyclic AMP Signaling. Mol Cell Biol. 2017 May 1;37(9)
• All: 8 reference(s)