Gt(ROSA)26Sor^{tm5(PTK2*Y397E*K454R)Khd}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm5(PTK2*Y397E*K454R)Khd}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 5, Kairbaan M Hodivala-Dilke
• MGI ID: MGI:5704298
• Synonyms: 397E/KD, R26FAK^KI
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm5(PTK2*Y397E*K454R)Khd} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:217505
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac or C57BL/6J

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm5(PTK2*Y397E*K454R)Khd} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  chicken	PTK2 (396416)

• Mutation details: A construct consisting of a floxed neo-stop cassette, chicken PTK2 cDNA with two mutations (Y397E and K454R) a Myc tag and an IRES-EGFP cassette was inserted into the locus. No GFP expression was detected. The mutations disrupt the kinase domain and mimic a constitutively phosphorylated form. (J:217505)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 958 strains or lines available

References

• Original: J:217505 Tavora B, et al., Generation of point-mutant FAK knockin mice. Genesis. 2014 Nov;52(11):907-15
• All: 2 reference(s)