Tg(Camk2a-rtTA2*M2)4Rsp
Transgene Detail

Summary

• Symbol: Tg(Camk2a-rtTA2*M2)4Rsp
• Name: transgene insertion 4, Rolf Sprenger
• MGI ID: MGI:5749403
• Synonyms: rTA-nM2^CaMK-4, Tg(CaMKII-rtTA2-nM2
• Transgene: Tg(Camk2a-rtTA2*M2)4Rsp Location: unknown
• Alliance: Tg(Camk2a-rtTA2*M2)4Rsp page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Transactivator)
• Mutation: Insertion
• Mutation details: The transgene expresses a novel tetracycline transactivator (tT) protein variant with a 13-amino acid SV40 nuclear localization signal (nls), optimized codons and, in place of the strong Herpes simplex VP16 activation domain, three ~14-amino acid minimal activation domains under the control of a forebrain-specific promoter fragment, comprising 8.5 kb of 5' flanking sequence and 84 base pairs of the first, noncoding exon, of the mouse calcium/calmodulin-dependent protein kinase II alpha (Camk2a) gene. This is one of four transgenic mouse lines with the SV40 nls that were generated. (J:129344)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:129344 Zhu P, et al., Silencing and un-silencing of tetracycline-controlled genes in neurons. PLoS One. 2007;2(6):e533
• All: 1 reference(s)