Loxl2^tm1.2Acan
Targeted Allele Detail

Summary

• Symbol: Loxl2^tm1.2Acan
• Name: lysyl oxidase-like 2; targeted mutation 1.2, Amparo Cano
• MGI ID: MGI:5750228
• Synonyms: L2^-
• Gene: Loxl2 Location: Chr14:69846517-69933283 bp, + strand Genetic Position: Chr14, 36.06 cM
• Alliance: Loxl2^tm1.2Acan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220689
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2 and a loxP site and FRT flanked neo cassette were inserted downstream of exon 2 via homologous recombination. Flp mediated recombination removed the neo cassette. Cre mediated recombination deleted exon 2. Semi-quantitative PCR and Western blot analysis of whole-cell extracts and conditioned medium confirmed the absence of transcript and protein expression in MEFs derived from homozygous mutant mice. (J:220689)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Loxl2 Mutation: 20 strains or lines available

References

• Original: J:220689 Martin A, et al., Lysyl oxidase-like 2 represses Notch1 expression in the skin to promote squamous cell carcinoma progression. EMBO J. 2015 Apr 15;34(8):1090-109
• All: 3 reference(s)