Gt(ROSA)26Sor^{tm1.1(Loxl2)Acan}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(Loxl2)Acan}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Amparo Cano
• MGI ID: MGI:5750303
• Synonyms: R26^L2
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(Loxl2)Acan} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220689
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1.1(Loxl2)Acan} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  Not Specified	Loxl2

• Mutation details: A floxed pgk-neo-STOP cassette, Loxl2 cDNA, IRES and EGFP were inserted via homologous recombination. Cre mediated recombination removed the pgk-neo-STOP cassette. Fluorescence imaging, semi-quantitative PCR, and Western blot analysis of whole-cell extracts and conditioned medium confirmed targeted overexpression in newborn homozygous mutant mice and derived MEFs. (J:220689)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:220689 Martin A, et al., Lysyl oxidase-like 2 represses Notch1 expression in the skin to promote squamous cell carcinoma progression. EMBO J. 2015 Apr 15;34(8):1090-109
• All: 3 reference(s)