Gt(ROSA)26Sor^{tm47(CAG-EGFP*)Hze}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm47(CAG-EGFP*)Hze}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 47, Hongkui Zeng
• MGI ID: MGI:5750793
• Synonyms: Ai47
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm47(CAG-EGFP*)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229154
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The Rosa-CAG targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), triple GFP reporter genes (emerald, TagGFP (Aequorea macrodactyla GFP), and hrGFP (Humanized Renilla reniformis GFP)), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. (J:229154)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1002 strains or lines available

References

• Original: J:229154 Allen Institute for Brain Science, TECHNICAL WHITE PAPER: TRANSGENIC CHARACTERIZATION. Allen Brain Atlas. 2015;9
• All: 15 reference(s)