Ightm3.1(VRC01)Nemz
Targeted Allele Detail
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Symbol: |
Ightm3.1(VRC01)Nemz |
Name: |
immunoglobulin heavy chain complex; targeted mutation 3.1, David Nemazee |
MGI ID: |
MGI:5779356 |
Synonyms: |
VRC01 gH |
Gene: |
Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic
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Germline Transmission: |
Earliest citation of germline transmission:
J:225518
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
C57BL/6
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Allele Type: |
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Targeted (Inserted expressed sequence) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A floxed neomycin resistance cassette replaced the DQ52-JH cluster in the Igh locus with the HIV broadly neutralizing antibody precursor VRC01 gH VDJ exon. The upstream promoter, leader and intron elements were from VHJ558.85.191. Cre-mediated recombination removed the neomycin resistance cassette.
(J:225518)
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Original: |
J:225518 Jardine JG, et al., HIV-1 VACCINES. Priming a broadly neutralizing antibody response to HIV-1 using a germline-targeting immunogen. Science. 2015 Jul 10;349(6244):156-61 |
All: |
7 reference(s) |
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