Exd1^tm1Embrp
Targeted Allele Detail

Summary

• Symbol: Exd1^tm1Embrp
• Name: exonuclease 3'-5' domain containing 1; targeted mutation 1, Ramesh Pillai
• MGI ID: MGI:5781095
• Gene: Exd1 Location: Chr2:119346986-119378108 bp, - strand Genetic Position: Chr2, 59.97 cM
• Alliance: Exd1^tm1Embrp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229314
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The construct inserted a three-frame triple stop codon cassette immediately downstream of the first codon in the exon 8 (ATG TAA A TAG A TGA), causing premature translation termination. A kanamycin-neomycin resistance gene cassette was inserted downstream of the stop codons. (J:229314)

Expression

In Mice Carrying this Mutation:

8 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Exd1 Mutation: 32 strains or lines available

References

• Original: J:229314 Yang Z, et al., PIWI Slicing and EXD1 Drive Biogenesis of Nuclear piRNAs from Cytosolic Targets of the Mouse piRNA Pathway. Mol Cell. 2016 Jan 7;61(1):138-52
• All: 2 reference(s)