Pik3cb^tm2.1Bvan
Targeted Allele Detail

Summary

• Symbol: Pik3cb^tm2.1Bvan
• Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta; targeted mutation 2.1, Bart Vanhaesebroeck
• MGI ID: MGI:5788280
• Synonyms: p110beta^D931A
• Gene: Pik3cb Location: Chr9:98920455-99022264 bp, - strand Genetic Position: Chr9, 51.41 cM, cytoband E4
• Alliance: Pik3cb^tm2.1Bvan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:228659
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting construct inserted after the STOP codon in the last exon (24) a loxP site, IRES sequence, lacZ reporter gene, neomycin resistance cassette and loxP site. Exon 22 was modified by nucleotide substitutions to accomplish the amino acid substitution of alanine for aspartic acid at position 931 (D931A). Cre-mediated recombination removed the reporter/selection cassette. (J:228659)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Pik3cb Mutation: 152 strains or lines available

References

• Original: J:228659 Guillermet-Guibert J, et al., Novel Role for p110beta PI 3-Kinase in Male Fertility through Regulation of Androgen Receptor Activity in Sertoli Cells. PLoS Genet. 2015 Jul;11(7):e1005304
• All: 1 reference(s)