Tg(tetO-TNF)15Wxlr
Transgene Detail

Summary

• Symbol: Tg(tetO-TNF)15Wxlr
• Name: transgene insertion 15, Viktor Wixler
• MGI ID: MGI:5804529
• Synonyms: ihTNFtg, Tg_ihTNFalphaF15, Tg(tetO-TNF)15
• Transgene: Tg(tetO-TNF)15Wxlr Location: unknown
• Alliance: Tg(tetO-TNF)15Wxlr page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-TNF)15Wxlr expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  TNF (7124)

  Tnf	(MGI:104798)

• Mutation details: This transgene contains the tetracycline-responsive P-tight promoter (from the plasmid pTRE-Tight, BD Biosciences Clontech), which comprises a modified Tet-responsive element containing seven direct repeats of a 36-bp sequence including the 19-bp tet operator sequence followed by a minimal cytomegalovirus (CMV) promoter, driving expression of a human tumor necrosis factor alpha cDNA whose 3' untranslated region (3'-UTR) has been replaced by the SV40 polyadenylation signal. This transgenic line was estimated by Southern blot analysis to carry 5 tandem copies of the transgene. No human TNFalpha is detected by enzyme-linked immunosorbent assay (ELISA) in sera of doxycycline-treated or untreated mice bearing this transgene alone. (J:236003)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:236003 Retser E, et al., Doxycycline-induced expression of transgenic human tumor necrosis factor alpha in adult mice results in psoriasis-like arthritis. Arthritis Rheum. 2013 Sep;65(9):2290-300
• All: 8 reference(s)