Card9^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Card9^{tm1c(EUCOMM)Hmgu}
• Name: caspase recruitment domain family, member 9; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5806258
• Synonyms: Card9^flox
• Gene: Card9 Location: Chr2:26242188-26250930 bp, - strand Genetic Position: Chr2, 18.87 cM
• Alliance: Card9^{tm1c(EUCOMM)Hmgu} page
• IMPC: Card9 gene page

Mutation origin

• Mutant Cell Line: HEPD0678_2_G09
• Germline Transmission: Earliest citation of germline transmission: J:235781
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 26246484 of Chromosome 2 upstream of exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 6 at position 26248459. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, with the deletion of the lacZ and neomycin sequences. Exons 3-6 are thus flanked by loxP sites. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:235781)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Card9 Mutation: 30 strains or lines available

References

• Original: J:235781 Nemeth T, et al., Neutrophil-specific deletion of the CARD9 gene expression regulator suppresses autoantibody-induced inflammation in vivo. Nat Commun. 2016;7:11004
• All: 3 reference(s)