Tg(Ins1-itTA)#Fto
Transgene Detail

Summary

• Symbol: Tg(Ins1-itTA)#Fto
• Name: transgene insertion, Francois Tronche
• MGI ID: MGI:5810641
• Synonyms: BAC-Ins1itTA, Ins1-itTA, Tg(BAC-Ins1-itTA), Tg(Ins1-tTA)Fto
• Transgene: Tg(Ins1-itTA)#Fto Location: unknown
• Alliance: Tg(Ins1-itTA)#Fto page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Transactivator)
• Mutation: Insertion
• Mutation details: MIce bearing this transgene express the improved-codon tetracycline transactivator (itTA) coding sequence under control of the promoter sequences of the mouse insulin 1 (Ins1) gene. The transgene was generated from the C57BL/6J-derived mouse bacterial artificial chromosome (BAC) clone RP23-401C13, containing the insulin 1 (Ins1) coding region and 110 kb and 87 kb of genomic DNA sequence upstream of its start codon and downstream of its polyadenylation signal, respectively. A cassette containing the itTA coding sequence followed by a single FRT site has replaced a 317-bp DNA segment beginning 3 bp upstream of the translation-initiating ATG of Ins1, disrupting the Ins1 open reading frame. X-gal staining of organ sections of mice carrying this and a second transgene expressing the E. coli lacZ gene under control of the tetracycline operator (tetO), in the absence of doxycycline, reveals beta-galactosidase expression that is restricted at embryonic day (E) 13.5 to the pancreatic buds, at E15 to the entire pancreas, and in the adult to insulin-expressing beta cells of the pancreatic islets. (J:223993)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

This transgene in combination with a second transgene whose expression is regulated by the tetracycline responsive element, comprising 7 copies of the tetracycline operator sequence (tetO) separated by spacer DNA sequences, functions as a Tet-Off system: When bound to doxycycline, the tTA protein represses transcription of the tetO-controlled target sequence.

References

• Original: J:223993 Blondeau B, et al., Novel transgenic mice for inducible gene overexpression in pancreatic cells define glucocorticoid receptor-mediated regulations of beta cells. PLoS One. 2012;7(2):e30210
• All: 1 reference(s)