Setdb2^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Setdb2^{tm1c(KOMP)Wtsi}
• Name: SET domain, bifurcated 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5816257
• Synonyms: Setdb2^ff
• Gene: Setdb2 Location: Chr14:59639458-59678329 bp, - strand Genetic Position: Chr14, 31.48 cM
• Alliance: Setdb2^{tm1c(KOMP)Wtsi} page
• IMPC: Setdb2 gene page

Mutation origin

• Mutant Cell Lines: EPD0164_4_B10, EPD0164_4_E09, EPD0164_4_E12
• Germline Transmission: Earliest citation of germline transmission: J:236763
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 59660574 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 59661423. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:236763)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Setdb2 Mutation: 53 strains or lines available

References

• Original: J:236763 Kroetz DN, et al., Type I Interferon Induced Epigenetic Regulation of Macrophages Suppresses Innate and Adaptive Immunity in Acute Respiratory Viral Infection. PLoS Pathog. 2015 Dec;11(12):e1005338
• All: 5 reference(s)