Gt(ROSA)26Sor^{tm1.1(CAG-AOX)Htj}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-AOX)Htj}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Howard T Jacobs
• MGI ID: MGI:5824337
• Synonyms: AOX^Rosa26, Gt(ROSA)26Sor^tm1.1Htj
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-AOX)Htj} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238705
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A construct comprising a CAG promotor, the ascidian Ciona intestinalis AOX (alternative oxidase) gene, a beta globin intron and a polyA site, plus an FLP site flanked neomycin resistance gene cassette were inserted into the locus. The neo cassette was removed through flp-mediated recombination. This creates an allele ubiquitously expressing an oxidase gene of a type not found in mammals. (J:238705)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:238705 Szibor M, et al., Broad AOX expression in a genetically tractable mouse model does not disturb normal physiology. Dis Model Mech. 2017 Feb 01;10(2):163-171
• All: 6 reference(s)