Mcmdc2^{tm4c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Mcmdc2^{tm4c(EUCOMM)Hmgu}
• Name: minichromosome maintenance domain containing 2; targeted mutation 4c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5892148
• Synonyms: Mcmdc2^restored
• Gene: Mcmdc2 Location: Chr1:9978863-10011179 bp, + strand Genetic Position: Chr1, 2.19 cM
• Alliance: Mcmdc2^{tm4c(EUCOMM)Hmgu} page
• IMPC: Mcmdc2 gene page

Mutation origin

• Mutant Cell Line: HEPD0781_2_C06
• Germline Transmission: Earliest citation of germline transmission: J:236226
• Parent Cell Line: JM8A3.N1.C2 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 9984471 of Chromosome 1 upstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 7 at position 9987378. Exons 5-7 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exons 5-7. (J:236226)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcmdc2 Mutation: 32 strains or lines available

References

• Original: J:236226 Finsterbusch F, et al., Alignment of Homologous Chromosomes and Effective Repair of Programmed DNA Double-Strand Breaks during Mouse Meiosis Require the Minichromosome Maintenance Domain Containing 2 (MCMDC2) Protein. PLoS Genet. 2016 Oct;12(10):e1006393
• All: 2 reference(s)