Smc3^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Smc3^{tm1c(EUCOMM)Wtsi}
• Name: structural maintenance of chromosomes 3; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5896795
• Synonyms: Smc3^fl
• Gene: Smc3 Location: Chr19:53588827-53634262 bp, + strand Genetic Position: Chr19, 48.13 cM
• Alliance: Smc3^{tm1c(EUCOMM)Wtsi} page
• IMPC: Smc3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:228976
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt1 cassette was inserted at position 53603082 of Chromosome 19 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of exon 4 at position 53603827. Exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 4. (J:228976)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Smc3 Mutation: 47 strains or lines available

References

• Original: J:228976 Viny AD, et al., Dose-dependent role of the cohesin complex in normal and malignant hematopoiesis. J Exp Med. 2015 Oct 19;212(11):1819-32
• All: 6 reference(s)