Mirc43^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mirc43^tm1Wtsi
• Name: microRNA cluster 43, including Mir341 through Mir1188; targeted mutation 2, Wellcome Trust Sanger Institute
• MGI ID: MGI:5898290
• Synonyms: Mir341-Mir1188 cluster 12N2 KO, Mir341^{tm1(mirKO)Wtsi}
• Gene: Mirc43 Location: Chr12:109552748-109578375 bp Genetic Position: Chr12, Syntenic

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_31B1, mirKO_ES_PuDtk_31B2, mirKO_ES_PuDtk_31B3, mirKO_ES_PuDtk_31B4, mirKO_ES_PuDtk_31B5, mirKO_ES_PuDtk_31B6, mirKO_ES_PuDtk_31B7, mirKO_ES_PuDtk_31B8, mirKO_ES_PuDtk_31B9, mirKO_ES_PuDtk_31B10, mirKO_ES_PuDtk_31B11, mirKO_ES_PuDtk_31B12
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: The cluster of microRNA genes was replaced via homologous recombination by a LoxP-F3-PGK-EM7-Purotk-bpA-LoxP-FRT targeting vector with 5.5kb (5') and 3.3kb (3') arms of homology. Briefly, this is a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters and flanked by LoxP and F3/FRT sites. The deletion starts at position 109611487 and ends at position 109611958 of Chromosome 12 (Genome Build 38). (J:94077, J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc43 Mutation: 1 strain or line available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 2 reference(s)