Tg(ACTB-Wld*)5Cole
Transgene Detail

Summary

• Symbol: Tg(ACTB-Wld*)5Cole
• Name: transgene insertion 5, Michael Coleman
• MGI ID: MGI:5911079
• Synonyms: deltaNLS^R213A,R215A Wld^S, deltaNLS Wld^S
• Transgene: Tg(ACTB-Wld*)5Cole Location: unknown
• Alliance: Tg(ACTB-Wld*)5Cole page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutations: Insertion, Nucleotide substitutions
• Tg(ACTB-Wld*)5Cole expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Wld (MGI:98952)	expresses Wld^s fusion gene with mutations that disable the NLS

• Mutation details: A construct consisting of the human beta actin promoter followed by a mutant cDNA for Wld^s that contains engineered C>G point mutations changing arginine codons 213 and 215 to alanine (p.Arg213Ala, p.Arg215Ala), was injected into the pronuclei of fertilized (C57BL/6 x CBA)F1 eggs that do not express Wld^s. The two mutations weaken or disable the nuclear localization signal, thus changing the localization of the translated peptide towards the cytoplasm. A small percentage of peptides is still directed to the nucleus. (J:144846)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:144846 Beirowski B, et al., Non-nuclear Wld(S) determines its neuroprotective efficacy for axons and synapses in vivo. J Neurosci. 2009 Jan 21;29(3):653-68
• All: 1 reference(s)