Gt(ROSA)26Sor^{tm1(CAG-EGFP*)Bpeng}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-EGFP*)Bpeng}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Bevin Engelward
• MGI ID: MGI:6114035
• Synonyms: RaDR-GFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-EGFP*)Bpeng} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:228545
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct contains a PGK-neo-pA cassette followed by a GFP direct repeat HR substrate. Specificly, the PGK-neo-pA cassette containes a single loxP site, the PGK promoter, a neomycin resistance gene and the PGK polyadenylation signal. The green fluorescent protein (GFP) direct repeat homologous recombination (HR) substrate consists of two CAG-DeltaEGFP-polyA expression cassettes arranged in tandem (Delta5egfp :: Delta3egfp). The Delta5egfp cassette has the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an enhanced green fluorescent protein missing 15 bp at its 5' end (Delta5egfp) and a rabbit beta-globin polyadenylation signal. The Delta3egfp cassette has the CAG hybrid promoter, an EGFP missing 81 bp at its 3' end (Delta3egfp) and a rabbit beta-globin polyadenylation signal followed by a bovine growth hormone polyadenylation sequence. The targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. (J:228545)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 958 strains or lines available

References

• Original: J:228545 Sukup-Jackson MR, et al., Rosa26-GFP direct repeat (RaDR-GFP) mice reveal tissue- and age-dependence of homologous recombination in mammals in vivo. PLoS Genet. 2014 Jun;10(6):e1004299
• All: 2 reference(s)