Mecr^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mecr^{tm1c(EUCOMM)Wtsi}
• Name: mitochondrial trans-2-enoyl-CoA reductase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6119418
• Gene: Mecr Location: Chr4:131570781-131595097 bp, + strand Genetic Position: Chr4, 64.52 cM
• Alliance: Mecr^{tm1c(EUCOMM)Wtsi} page
• IMPC: Mecr gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:242847
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt2 cassette was inserted at position 131580788 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 131581364. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:155845)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mecr Mutation: 17 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 3 reference(s)