Prdm1^tm2Mbu
Targeted Allele Detail

Summary

• Symbol: Prdm1^tm2Mbu
• Name: PR domain containing 1, with ZNF domain; targeted mutation 2, Meinrad Busslinger
• MGI ID: MGI:6149541
• Synonyms: Prdm1^ihCd2-Neo
• Gene: Prdm1 Location: Chr10:44313173-44404497 bp, - strand Genetic Position: Chr10, 23.24 cM
• Alliance: Prdm1^tm2Mbu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:259465
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: A FLAG-V5-TEV cleavage site-biotin tag construct, an FRT flanked IRES-CD2 reporter construct and a loxP flanked pgk-neo cassette were inserted in the end of exon 8 via homologous recombination. This allele was used to create Prdm1^tm1Mbu (J:259465)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prdm1 Mutation: 64 strains or lines available

References

• Original: J:259465 Minnich M, et al., Multifunctional role of the transcription factor Blimp-1 in coordinating plasma cell differentiation. Nat Immunol. 2016 Mar;17(3):331-43
• All: 2 reference(s)