Rr170^tm5.1Iku
Targeted Allele Detail

Summary

• Symbol: Rr170^tm5.1Iku
• Name: regulatory region 170; targeted mutation 5.1, Koichi Ikuta
• MGI ID: MGI:6151131
• Synonyms: Il7rCNS1.GRE12m, Il7r^tm5.1Iku
• Gene: Rr170 Location: unknown Genetic Position: Chr15, Syntenic
• Alliance: Rr170^tm5.1Iku page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:260412
• Parent Cell Line: KY1.1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutation: Nucleotide substitutions
• Mutation details: Mice harboring point mutations in both glucocorticoid response elements (GREs) in the Il7r enhancer (upstream of the gene) were generated. The sequence mutated is in the first element (GRE2) as follows: GRE1 wild-type, 5-CTTTGTTCTTTTACATCTTCA-3; GRE1m, 5-CTTcacTgcTTTgagTgcTCA-3; GRE2 wild-type, 5-TAGCACATGCTGTACC-3; GRE2m, 5-TAGCgagTGCTGTACC-3. A targeting construct containing the mutations and a loxP site flanked neomycin selection cassette was used to create mutant ES cells via homologous recombination. The neo cassette was removed through subsequent cre-mediated recombination. (J:260412)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr170 Mutation: 0 strains or lines available

References

• Original: J:260412 Shimba A, et al., Glucocorticoids Drive Diurnal Oscillations in T Cell Distribution and Responses by Inducing Interleukin-7 Receptor and CXCR4. Immunity. 2018 Feb 20;48(2):286-298.e6
• All: 1 reference(s)