C1qa^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: C1qa^{tm1d(EUCOMM)Wtsi}
• Name: complement component 1, q subcomponent, alpha polypeptide; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6151469
• Gene: C1qa Location: Chr4:136623228-136626114 bp, - strand Genetic Position: Chr4, 69.05 cM
• Alliance: C1qa^{tm1d(EUCOMM)Wtsi} page
• IMPC: C1qa gene page

Mutation origin

• Mutant Cell Line: EPD0618_1_B03
• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at a position on Chromosome 4 upstream of exon 3 of the gene. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted in the 3' untranslated region of exon 3. Flp-mediated recombination removed the FRT-flanked lacZ and neo cassette. Cre-mediated recombination removed exon 3. (J:240681)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any C1qa Mutation: 24 strains or lines available

References

• Original: J:240681 Fonseca MI, et al., Cell-specific deletion of C1qa identifies microglia as the dominant source of C1q in mouse brain. J Neuroinflammation. 2017 Mar 06;14(1):48
• All: 20 reference(s)