Btk^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Btk^{tm1c(EUCOMM)Hmgu}
• Name: Bruton agammaglobulinemia tyrosine kinase; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6155006
• Synonyms: Btk^flox
• Gene: Btk Location: ChrX:133443085-133484319 bp, - strand Genetic Position: ChrX, 56.18 cM
• Alliance: Btk^{tm1c(EUCOMM)Hmgu} page
• IMPC: Btk gene page

Mutation origin

• Mutant Cell Line: HEPD0522_1_A11
• Germline Transmission: Earliest citation of germline transmission: J:261314
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 133459215 of Chromosome X upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 133460544. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:261314)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Btk Mutation: 21 strains or lines available

References

• Original: J:261314 Nyhoff LE, et al., Bruton's Tyrosine Kinase Is Not Essential for B Cell Survival beyond Early Developmental Stages. J Immunol. 2018 Apr 1;200(7):2352-2361
• All: 6 reference(s)