Tg(Thy1-KCNB1*C73A)18Sesf
Transgene Detail

Summary

• Symbol: Tg(Thy1-KCNB1*C73A)18Sesf
• Name: transgene insertion 18, Federico Sesti
• MGI ID: MGI:6160085
• Synonyms: Tg-C73A
• Transgene: Tg(Thy1-KCNB1*C73A)18Sesf Location: unknown
• Alliance: Tg(Thy1-KCNB1*C73A)18Sesf page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Thy1-KCNB1*C73A)18Sesf expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  KCNB1 (3745)

  Kcnb1

  (MGI:96666)

  expresses a non-oxidizable mutant protein

• Mutation details: The transgenic construct was generated by cloning into the Thy1.2 expression cassette a cDNA encoding a nonoxidizable variant, in which alanine is substituted for cysteine at amino acid position 73 (C73A), of human potassium voltage-gated channel subfamily B member 1 (KCNB1) with a C-terminal human influenza hemagglutinin (HA) tag.

  Immunohistologic analysis of coronal sections of adult mice using antibodies against the HA tag of the transgene-derived human KCNB1 and against a conserved C-terminal epitope common to mouse and human KCNB1 demonstrate expression of transgenic and total (mouse and human in transgenic or mouse only in control mice) KCNB1 in the cortex and hippocampus, but not other brain regions. Both antibodies reveal clustered KCNB1 channels in plasma membranes of cortical and hippocampal neurons. Densitometric analysis of immunoblots of brain lysates probed with the non-specific antibody shows that transgenic brains contain approximately approximately 1.5-fold the number of channels present in non-transgenic control lysates, or half as many transgene-derived as endogenous channels. (J:260029)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Four founder mice bearing C73A mutant KCNB1 transgenes were produced. Results are presented for line #18. (J:260029, J:260051)

References

• Original: J:260029 Yu W, et al., Oxidation of KCNB1 Potassium Channels Causes Neurotoxicity and Cognitive Impairment in a Mouse Model of Traumatic Brain Injury. J Neurosci. 2016 Oct 26;36(43):11084-11096
• All: 4 reference(s)