Acan^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Acan^{tm1c(EUCOMM)Hmgu}
• Name: aggrecan; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6160877
• Synonyms: Acan-loxP
• Gene: Acan Location: Chr7:78703231-78764847 bp, + strand Genetic Position: Chr7, 44.88 cM
• Alliance: Acan^{tm1c(EUCOMM)Hmgu} page
• IMPC: Acan gene page

Mutation origin

• Mutant Cell Lines: HEPD0602_5_F12, HEPD0602_5_G11
• Germline Transmission: Earliest citation of germline transmission: J:267250
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: Flp-mediated recombination of the parental Acan^{tm1a(EUCOMM)Hmgu} allele excised the splice acceptor-lacZ reporter sequence and the neomycin selection cassette from the third intron, leaving the critical exon 4 flanked by loxP sites. Exposure to Cre recombinase will delete exon 4, resulting in a null mutation. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:267250)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Acan Mutation: 80 strains or lines available

References

• Original: J:267250 Rowlands D, et al., Aggrecan Directs Extracellular Matrix-Mediated Neuronal Plasticity. J Neurosci. 2018 Nov 21;38(47):10102-10113
• All: 2 reference(s)