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Tg(Tph2-aop3*,-EGFP)12Gfng
Transgene Detail
Summary
Symbol: Tg(Tph2-aop3*,-EGFP)12Gfng
Name: transgene insertion 12, Guoping Feng
MGI ID: MGI:6187689
Synonyms: Tph2-eArchT3.0-EGFP
Transgene: Tg(Tph2-aop3*,-EGFP)12Gfng  Location: unknown  
Alliance: Tg(Tph2-aop3*,-EGFP)12Gfng page
Transgene
origin
Strain of Origin:  C57BL/6
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsBAC RP23-112F24 was used to obtain the entire Tph2 coding sequence; the following was inserted in-frame immediately after the Tph2 start codon: an eArchT3.0 cDNA sequence (described further below), a viral 2A oligopeptide sequence, an enhanced green fluorescent protein (EGFP), a woodchuck hepatitis virus posttranscriptional regulatory element (WPRE; to enhance mRNA stability) and a bovine growth hormone polyadenylation signal (BGHpA) The eArchT3.0 sequence is a mammalian codon-optimized cDNA encoding ArchT (the Archaerhodopsin-3 protein [aop3] derived from the halophilic bacterium Halorubrum strain TP009) that was further modified to have a neurite/membrane-trafficking signal (TS) sequence (amino acids GEYIPLDQIDINV) and an endoplasmic reticulum (ER) exporting sequence (amino acids FCYENEV). (J:341572)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:341572 Krol A, et al., Two eARCHT3.0 Lines for Optogenetic Silencing of Dopaminergic and Serotonergic Neurons. Front Neural Circuits. 2019;13:4
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
06/12/2024
MGI 6.13
The Jackson Laboratory