Gt(ROSA)26Sor^{tm1.1(CAG-Cerulean/Arl13b,-Venus/GMNN,-Cherry/CDT1)Rmort}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-Cerulean/Arl13b,-Venus/GMNN,-Cherry/CDT1)Rmort}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Richard Mort
• MGI ID: MGI:6193734
• Synonyms: R26Arl13b-Fucci2a
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-Cerulean/Arl13b,-Venus/GMNN,-Cherry/CDT1)Rmort} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:267034
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: Sequences include CMV enhancer/chicken beta-actin promoter (CAG), a loxP-STOP(3xSV40 polyA)-loxP cassette (LSL), full-length mouse Arl13b cDNA (OTTMUST00000058920.GRCm38) fused to mCerulean and Fucci2a using the porcine teschovirus-1 2A (P2A) ribosomal skip cleavage peptide sequence on the N-terminus of the Fucci2a cell cycle probe consisting of a fusion of mCherry with a truncated human CDT1 (aa 30-120) fused to mCherry and mVenus fused to 110aa N-terminal human Geminin fragmentand a bovine growth hormone polyA sequence (BGHpA). Cre-mediated recombination removed the STOP cassette. (J:267034)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:267034 Ford MJ, et al., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell. 2018 Nov 19;47(4):509-523.e5
• All: 1 reference(s)