Tg(tetO-LINE1*,-lacZ)#Ggs
Transgene Detail

Summary

• Symbol: Tg(tetO-LINE1*,-lacZ)#Ggs
• Name: transgene insertion, Gerald G Schumann
• MGI ID: MGI:6220704
• Synonyms: Tg(TRE-ORFeus,-lacZ)
• Transgene: Tg(tetO-LINE1*,-lacZ)#Ggs Location: unknown
• Alliance: Tg(tetO-LINE1*,-lacZ)#Ggs page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: A bidirectional tetracycline/doxycycline- (tet/dox-) responsive promoter drives expression in one direction of the E. coli lacZ (beta-galactosidase) gene, and in the other direction of a LINE1 retrotransposition reporter cassette ("ORFeus"). The ORFeus cassette includes 1) the codon-optimized open reading frames (ORF1 and ORF2) of a human LINE1 (long interspersed nuclear element 1), encoding the proteins required for its retrotransposition; and 2) a green fluorescent protein (GFP) reporter gene, interrupted by an intron, inserted in reverse orientation into the LINE1 3'-untranslated region (3'-UTR). lacZ expression serves as a reporter of tet/dox-induced transcription, and excision of the intron from the ORFeus transcript permits expression of GFP from a retrotransposed copy. (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)