Pum2^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pum2^{tm1d(KOMP)Wtsi}
• Name: pumilio RNA-binding family member 2; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6272003
• Synonyms: Pum2^-
• Gene: Pum2 Location: Chr12:8724134-8802581 bp, + strand Genetic Position: Chr12, 3.92 cM, cytoband A1.3
• Alliance: Pum2^{tm1d(KOMP)Wtsi} page
• IMPC: Pum2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:268040
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 8762870 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 8764206. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:268040)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pum2 Mutation: 104 strains or lines available

References

• Original: J:268040 Lin K, et al., Generation and functional characterization of a conditional Pumilio2 null allele. J Biomed Res. 2018 Nov 20;32(5):434-441
• All: 2 reference(s)